N-Cadherin Antikörper (Middle Region)

Produktnummer ABIN3044370

Produktdetails anti-N-Cadherin Antikörper

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Bindungsspezifität: AA 701-714, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Cadherin-2(CDH2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: CQCDSNGDCT DVDR

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cadherin-2(CDH2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cadherin 2, type 1, N-cadherin(neuronal)
Protein Name: Cadherin-2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin(701-714aa CQCDSNGDCTDVDR), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-N-Cadherin Antikörper (ABIN3044370)

Inoue, Takemori, Mizuguchi, Kimura, Chikugo, Hagiyama, Yoneshige, Mori, Maenishi, Kometani, Itoh, Satou, Ito: "Heart-bound adiponectin, not serum adiponectin, inversely correlates with cardiac hypertrophy in stroke-prone spontaneously hypertensive rats." in: Experimental physiology, Vol. 102, Issue 11, pp. 1435-1447, (2018) (PubMed).

Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

Jia, Jiang, Liu, Wang, Zhu, Zhu, Liu, Zhong, Xie, Huang, Jia, Li, Liu, Zuo, Cheng, Dai, Ren: "Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells." in: International journal of oncology, Vol. 52, Issue 6, pp. 1787-1800, (2018) (PubMed).

Shen, Cai, Liu, Li, Gan, Li, Wang, Guo, Zhou, Lu, Sun, Li: "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." in: Journal of hematology & oncology, Vol. 11, Issue 1, pp. 95, (2018) (PubMed).

Pan, An, Meng, Li, Ren, Guan: "MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4663-4670, (2017) (PubMed).

Wan, Tian, Qi, Liu, Xu: "ANXA1 affects cell proliferation, invasion and epithelial-mesenchymal transition of oral squamous cell carcinoma." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 5214-5218, (2017) (PubMed).

Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, (2015) (PubMed).

Liu, Liu, Jiang, Peng, Ma, Lu: "SOX9 Overexpression Promotes Glioma Metastasis via Wnt/?-Catenin Signaling." in: Cell biochemistry and biophysics, (2015) (PubMed).

Sun, Hu, Zheng, Bagnoli, Guo, Rupaimoole, Rodriguez-Aguayo, Lopez-Berestein, Ji, Chen, Sood, Mezzanzanica, Liu, Sun, Zhang: "MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer." in: The Journal of pathology, Vol. 235, Issue 1, pp. 25-36, (2015) (PubMed).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, (2015) (PubMed).

Yang, Hui, Wang, Yang, Jiang: "Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/?-catenin pathway in laryngeal cancer Hep-2 cells." in: Tumour biology, Vol. 35, Issue 8, pp. 7965-73, (2014) (PubMed).

Kudo-Sakamoto, Akazawa, Ito, Takano, Yano, Yabumoto, Naito, Oka, Lee, Sakata, Suzuki, Saido, Komuro: "Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling." in: The Journal of biological chemistry, Vol. 289, Issue 28, pp. 19408-19, (2014) (PubMed).

Wang, Li, Zhou, Sun, Wang, Cheng, Ma, Liu, Liu, Wang, Liang, Wu: "Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin." in: PLoS ONE, Vol. 8, Issue 4, pp. e61687, (2013) (PubMed).

Qiao, Johnson, Gao et al.: "Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-beta1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 ..." in: International journal of oncology, Vol. 37, Issue 3, pp. 663-8, (2010) (PubMed).

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: CDH2 (CDH2 Produkte)

Synonyme: CD325 antikoerper, CDHN antikoerper, CDw325 antikoerper, NCAD antikoerper, N-cadherin antikoerper, cadherin-2 antikoerper, cdhn antikoerper, ncad antikoerper, cdw325 antikoerper, CDH2 antikoerper, Cadherin-2 antikoerper, Ncad antikoerper, glo antikoerper, lyr antikoerper, pac antikoerper, wu:fb47h04 antikoerper, cadherin 2 antikoerper, cadherin 2 S homeolog antikoerper, cadherin 2, type 1, N-cadherin (neuronal) antikoerper, CDH2 antikoerper, cdh2.S antikoerper, cdh2 antikoerper, Cdh2 antikoerper

Hintergrund: N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96 % identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Synonyms: CADH2_HUMAN antibody|Cadherin 2 antibody|Cadherin 2 N cadherin neuronal antibody|Cadherin 2 type 1 antibody|Cadherin 2 type 1 N cadherin neuronal antibody|cadherin 2 type 1 N-cadherin neuronal antibody|Cadherin 2, type 1, N-cadherin(neuronal) antibody|Cadherin-2 antibody|Cadherin2 antibody|Calcium dependent adhesion protein neuronal antibody|CD325 antibody|CD325 antigen antibody|CDH2 antibody|CDHN antibody|CDw325 antibody|CDw325 antigen antibody|N cadherin 1 antibody|N-cadherin antibody|NCAD antibody|Neural Cadherin antibody|OTTHUMP00000066304 antibody|OTTHUMP00000067378 antibody

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane