NOS1 Antikörper (C-Term)

Produktnummer ABIN3044371

Produktdetails anti-NOS1 Antikörper

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Bindungsspezifität: AA 1418-1434, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Nitric oxide synthase, brain(NOS1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: IAFIEESKKD TDEVFSS

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Nitric oxide synthase, brain(NOS1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: nitric oxide synthase 1(neuronal)
Protein Name: Nitric oxide synthase, brain

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal)(1418-1434aa IAFIEESKKDTDEVFSS), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-NOS1 Antikörper (ABIN3044371)

Yin, Liang, Wang, Yan, Yin, Wu, Su: "Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 649-658, (2018) (PubMed).

Ding, Tang, Tang, Zhang, Wang: "A Variant in the Precursor of MicroRNA-146a is Responsible for Development of Erectile Dysfunction in Patients with Chronic Prostatitis via Targeting NOS1." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 929-937, (2017) (PubMed).

Zheng, Sulieman, Li, Wei, Xu, Shi: "Nitric oxide and thyroid hormone receptor alpha 1 contribute to ovarian follicular development in immature hyper- and hypo-thyroid rats." in: Reproductive biology, Vol. 15, Issue 1, pp. 27-33, (2015) (PubMed).

Kong, Wei, Fedail, Shi, Nagaoka, Watanabe: "Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats." in: The Journal of reproduction and development, Vol. 61, Issue 3, pp. 219-27, (2015) (PubMed).

Li, Zhou, Wei, Huang, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase ? and ? subunits in postnatal porcine uteri." in: Acta histochemica, Vol. 116, Issue 3, pp. 466-73, (2014) (PubMed).

Xu, Wei, Zheng, Mao, Zheng, Li, Shi: "Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress." in: Acta histochemica, Vol. 116, Issue 8, pp. 1323-30, (2014) (PubMed).

Liu, Li, Zhang, Fu, Liu, Zhou: "Activity and expression of nitric oxide synthase in pork skeletal muscles." in: Meat science, Vol. 99, pp. 25-31, (2014) (PubMed).

Fedail, Zheng, Wei, Kong, Shi: "Roles of thyroid hormones in follicular development in the ovary of neonatal and immature rats." in: Endocrine, Vol. 46, Issue 3, pp. 594-604, (2014) (PubMed).

Ding, Zhang, Hui, Zhang, Zhang, Li, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase ?1 and ?1 subunits in the ovary of fetal, neonatal and immature pigs." in: Animal reproduction science, Vol. 131, Issue 3-4, pp. 172-80, (2012) (PubMed).

Chang, Xiao, Wang, Yan, Yang, Wang: "2, 3, 5, 4'-Tetrahydroxystilbene-2-O-beta-D-glucoside improves gastrointestinal motility disorders in STZ-induced diabetic mice." in: PLoS ONE, Vol. 7, Issue 12, pp. e50291, (2012) (PubMed).

Zhang, Wei, Wang, Ding, Wang, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats." in: Journal of Zhejiang University. Science. B, Vol. 12, Issue 1, pp. 55-64, (2011) (PubMed).

Wang, Yan, Zhao, Wu, Zhou: "The diversity of nNOS gene expression in avulsion-injured spinal motoneurons among laboratory rodents." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 22, Issue 1, pp. 37-42, (2010) (PubMed).

Wu, Yin, Yao, Haefliger: "Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro." in: Molecular vision, Vol. 13, pp. 493-6, (2007) (PubMed).

Yuan, Liu, Zhang, Yuan, Muteliefu, Lu: "Upregulated expression of neuronal nitric oxide synthase by insulin in both neurons and astrocytes." in: Brain research, Vol. 1008, Issue 1, pp. 1-10, (2004) (PubMed).

Chen, Qian, Zhang, Chang, Duan: "Distribution of constitutive nitric oxide synthase in the jejunum of adult rat." in: World journal of gastroenterology, Vol. 8, Issue 3, pp. 537-9, (2002) (PubMed).

Details zu NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Andere Bezeichnung: NOS1 (NOS1 Produkte)

Synonyme: NOS1 antikoerper, IHPS1 antikoerper, N-NOS antikoerper, NC-NOS antikoerper, NOS antikoerper, bNOS antikoerper, nNOS antikoerper, 2310005C01Rik antikoerper, NO antikoerper, NOS-I antikoerper, Nos-1 antikoerper, nos antikoerper, ihps1 antikoerper, nnos antikoerper, nos1 antikoerper, nos1-A antikoerper, nanos C2HC-type zinc finger 1 antikoerper, nitric oxide synthase 1 antikoerper, nitric oxide synthase 1, neuronal antikoerper, nitric oxide synthase 1 (neuronal) antikoerper, nitric oxide synthase 1 L homeolog antikoerper, NANOS1 antikoerper, NOS1 antikoerper, Nos1 antikoerper, nos1 antikoerper, nos1.L antikoerper

Hintergrund: Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

Synonyms: 2310005C01Rik antibody|bNOS antibody|Constitutive NOS antibody|EC 1.14.13.39 antibody|IHPS 1 antibody|IHPS1 antibody|N NOS antibody|N-NOS antibody|NC NOS antibody|NC-NOS antibody|neuronal Nitric Oxide Synthase antibody|Neuronal NOS antibody|Nitric oxide synthase, neuronal, included antibody|Nitric oxide synthase 1(neuronal) antibody|Nitric oxide synthase 1 antibody|Nitric oxide synthase brain antibody|Nitric oxide synthase, brain antibody|Nitric oxide synthase, penile neuronal, included antibody|nNOS antibody|NO antibody|NOS 1 antibody|NOS antibody|NOS type I antibody|NOS-I antibody|NOS1 antibody|NOS1_HUMAN antibody|Peptidyl-cysteine S-nitrosylase NOS1 antibody

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction