GAD65 Antikörper (N-Term)
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- Target Alle GAD65 (GAD2) Antikörper anzeigen
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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Bindungsspezifität
- AA 21-37, N-Term
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Reaktivität
- Human, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GAD65 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequenz
- ENPGTARAWC QVAQKFT
- Kreuzreaktivität (Details)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Produktmerkmale
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Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glutamate decarboxylase 2(pancreatic islets and brain, 65 kDa)
Protein Name: Glutamate decarboxylase 2 - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.
- Isotyp
- IgG
- Top Product
- Discover our top product GAD2 Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Konservierungsmittel
- Thimerosal (Merthiolate), Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Haltbarkeit
- 12 months
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Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, (2015) (PubMed).
: "Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum." in: Toxicology and industrial health, Vol. 28, Issue 1, pp. 10-20, (2012) (PubMed).
: "
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Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, (2015) (PubMed).
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- Target
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Andere Bezeichnung
- GAD2 (GAD2 Produkte)
- Synonyme
- GAD65 antikoerper, gad65 antikoerper, F12P19.12 antikoerper, F12P19_12 antikoerper, GLUTAMATE DECARBOXYLASE 2 antikoerper, glutamate decarboxylase 2 antikoerper, zgc:112198 antikoerper, 6330404F12Rik antikoerper, GAD(65) antikoerper, Gad-2 antikoerper, glutamate decarboxylase 2 antikoerper, glutamate decarboxylase 1 antikoerper, glutamic acid decarboxylase 2 antikoerper, GAD2 antikoerper, PTRG_06148 antikoerper, LOC9324534 antikoerper, Gad2 antikoerper, GAD1 antikoerper, gad2 antikoerper
- Hintergrund
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Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Synonyms: 65 kDa glutamic acid decarboxylase antibody|DCE 2 antibody|DCE2 antibody|DCE2_HUMAN antibody|GAD 2 antibody|GAD 65 antibody|GAD-2 antibody|GAD-65 antibody|Gad2 antibody|Glutamate decarboxylase 2(pancreas) antibody|Glutamate Decarboxylase 2(pancreatic islets and brain 65 kDa) antibody|Glutamate decarboxylase 2 antibody|Glutamate Decarboxylase 65 antibody|Glutamate decarboxylase 65 kDa isoform antibody|Glutamic Acid Decarboxylase 2 antibody|Glutamic Acid Decarboxylase 65 antibody|MGC161605 antibody|MGC161607 antibody - UniProt
- Q05329
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