Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Aufreinigung
Protein G purified
Immunogen
PAX6 transcription factor linked to histidine tag.
WB, ELISA, IP, DB, ISt
Wirt: Maus
Monoclonal
HIS-H8
unconjugated
Applikationshinweise
Approved: Flo (1:10), IHC
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
PBS, 0.09 % sodium azide, 1 % BSA.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.