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Claudin 12 Antikörper (N-Term)

CLDN12 Reaktivität: Human, Maus WB, IHC, IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN305786
  • Target Alle Claudin 12 (CLDN12) Antikörper anzeigen
    Claudin 12 (CLDN12)
    Bindungsspezifität
    • 21
    • 8
    • 7
    • 6
    • 3
    • 1
    N-Term
    Reaktivität
    • 31
    • 19
    • 16
    Human, Maus
    Wirt
    • 44
    • 3
    Kaninchen
    Klonalität
    • 45
    • 2
    Polyklonal
    Konjugat
    • 12
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Claudin 12 Antikörper ist unkonjugiert
    Applikation
    • 33
    • 28
    • 23
    • 13
    • 13
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Marke
    IHC-plus™
    Spezifität
    Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
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  • Applikationshinweise
    Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS,  pH 7.4, 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Aliquot to Avoid freeze/thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    Claudin 12 (CLDN12)
    Andere Bezeichnung
    CLDN12 / Claudin 12 (CLDN12 Produkte)
    Synonyme
    CLDN12 antikoerper, Claudin-12 antikoerper, DKFZp469F1620 antikoerper, zgc:86588 antikoerper, wu:fc27c11 antikoerper, wu:fc37h01 antikoerper, claudin-12 antikoerper, RGD1561053 antikoerper, claudin 12 antikoerper, claudin 12 S homeolog antikoerper, CLDN12 antikoerper, cldn12 antikoerper, Cldn12 antikoerper, cldn12.S antikoerper, CpipJ_CPIJ011954 antikoerper
    Hintergrund
    Name/Gene ID: CLDN12
    Family: Claudin

    Synonyms: CLDN12, Claudin 12, Claudin-12
    Gen-ID
    9069
    UniProt
    P56749
    Pathways
    Cell-Cell Junction Organization
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