Phosphotyrosine Antikörper (FITC)

Produktnummer ABIN306701

Produktdetails anti-Phosphotyrosine Antikörper (FITC)

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktmerkmale: Mouse anti phosphotyrosine, PY20, FITC conjugate,The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Aufreinigung: Prot A/G

Immunogen: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Klon: PY20

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: A dilution of 5-10 μg/mL is suggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as sterile solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol, 3 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Produkte

Substanzklasse: Amino Acid