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Phosphotyrosine Antikörper (Peroxidase (POD))

WB, IP, IHC, EIA Wirt: Maus Monoclonal PY20 Peroxidase (POD)

Produktnummer ABIN306704

Datenblatt (PDF)

Target Alle Phosphotyrosine Produkte

Phosphotyrosine

Reaktivität

Bitte anfragen
Wirt
89

28

1
Maus
Passende Sekundärantikörper
Klonalität
100

18
Monoklonal
Konjugat
38

17

11

8

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Phosphotyrosine Antikörper ist konjugiert mit Peroxidase (POD)
Applikation
81

61

40

39

34

23

22

17

13

9

7

7

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Produktmerkmale

Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate,The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years, While the phosphorylation of specific tyrosine residues has be shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved invaluable to the study of tyrosine-phosphorylated proteins and the biochemical pathways in which they fuction. The HRP conjugate of PY20 anti-phosphotyrosine is especailly useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immuncytochemical protcols in situations wherin the use of a secondary antibody would produce undesirable background signals.

Aufreinigung

Prot A/G

Immunogen

Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Klon

PY20

Isotyp

IgG2b
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Phosphotyrosine antibody
Reaktivität: Diverse Spezies WB, FACS, ICC Wirt: Maus Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Wirt: Maus Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reaktivität: Diverse Spezies WB, IHC (p) Wirt: Maus Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM Wirt: Maus Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 FITC

anti-Phosphotyrosine antibody (Biotin)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 Biotin

anti-Phosphotyrosine antibody (PE)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 PE

Applikationshinweise

Working dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Buffer

Provided in 60 % glycerol, phosphate buffered saline, and 0.1 % 2-Chloroacetamide

Lagerung

-20 °C

Informationen zur Lagerung

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Target

Phosphotyrosine

Abstract

Phosphotyrosine Produkte

Substanzklasse

Amino Acid