-
- Target
- Farnesyl
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Unkonjugiert
- Applikation
- Immunofluorescence (IF), ELISA
- Produktmerkmale
- Protein isoprenylation is a post-translational modification that affects about 0.5 % of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.
- Aufreinigung
- Ammonium Sulfate Precipitation
- Immunogen
- Rabbit immunized with farnesyl cysteine conjugated to KLH.
- Isotyp
- IgG
-
anti-Farnesyl antibody
IF, EIA Wirt: Kaninchen Polyclonal unconjugated
-
- Applikationshinweise
- Optimal concentration should be evaluated by serial dilutions.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Buffer
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
-
- Target
- Farnesyl
- Substanzklasse
- Chemical
- UniProt
- P14324
-
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