Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
Kreuzreaktivität
Human, Maus, Ratte
Produktmerkmale
Myogenin, Myogenic factor 4, Myf-4,Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Aufreinigung
Protein A/G Chromatography
Immunogen
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 μg/mg), Western blotting (1 μg/mL) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide,X1415M, clone F12B recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles