BrdU Antikörper (FITC)

Produktnummer ABIN306900

Produktdetails anti-BrdU Antikörper (FITC)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist konjugiert mit FITC

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktmerkmale: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody is not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.

Aufreinigung: Protein A/G Chromatography

Immunogen: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin was used to immunize BALB/c mice

Klon: MoBU-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Reacts with cells containing incorporated BrdU, showing a clear, nucleus confined, speckled pattern. Use at 1-5 μg/106 cells for flow cytometry. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU (bromodeoxyuridine) (BrdU Produkte)

Substanzklasse: Chemical