Cathepsin G Antikörper (Internal Region)
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- Target Alle Cathepsin G (CTSG) Antikörper anzeigen
- Cathepsin G (CTSG)
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Cathepsin G Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.
- Produktmerkmale
- Rabbit Polyclonal to Cathepsin G.
- Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from the Internal region of human Cathepsin G.
- Isotyp
- IgG
- Top Product
- Discover our top product CTSG Primärantikörper
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- Applikationshinweise
- WB 1:500-1:2000, ELISA 1:40000,
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- NanoTemper Technologies
- No.
- #102721
- Datum
- 05.11.2018
- Antigen
- CTSG
- Chargennummer
- 022204892
- Validierte Anwendung
- Unfolding Profile
- Positivkontrolle
- ABIN3183694
- Negativkontrolle
- Bewertung
Passed. ABIN3183694 showed Ti at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.
- Primärantikörper
- ABIN3183694
- Sekundärantikörper
- Full Protocol
- Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Anmerkungen
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
Validierung #102721 (Unfolding Profile)Validierungsbilderp>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.Protokoll -
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C.
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- Target
- Cathepsin G (CTSG)
- Andere Bezeichnung
- Cathepsin G (CTSG Produkte)
- Synonyme
- CATG antikoerper, CG antikoerper, CTSG antikoerper, Ctsg antikoerper, cathepsin G antikoerper, CTSG antikoerper, Ctsg antikoerper, LOC505658 antikoerper, LOC509956 antikoerper, ctsg antikoerper, LOC100053921 antikoerper, LOC102174648 antikoerper, LOC100733619 antikoerper, LOC100154047 antikoerper
- Molekulargewicht
- 22 kDa
- Gen-ID
- 1511
- UniProt
- P08311
- Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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