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Cathepsin G Antikörper (Internal Region)

CTSG Reaktivität: Human WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3183694
  • Target Alle Cathepsin G (CTSG) Antikörper anzeigen
    Cathepsin G (CTSG)
    Bindungsspezifität
    • 16
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 51
    • 28
    • 21
    • 2
    Human
    Wirt
    • 61
    • 3
    Kaninchen
    Klonalität
    • 62
    • 2
    Polyklonal
    Konjugat
    • 30
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Cathepsin G Antikörper ist unkonjugiert
    Applikation
    • 46
    • 15
    • 13
    • 13
    • 13
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    Western Blotting (WB), ELISA
    Spezifität
    Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.
    Produktmerkmale
    Rabbit Polyclonal to Cathepsin G.
    Aufreinigung
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    Synthesized peptide derived from the Internal region of human Cathepsin G.
    Isotyp
    IgG
    Top Product
    Discover our top product CTSG Primärantikörper
  • Applikationshinweise
    WB 1:500-1:2000, ELISA 1:40000,
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #102721 (Unfolding Profile)
    'Independent Validation' Siegel
    by
    NanoTemper Technologies
    No.
    #102721
    Datum
    05.11.2018
    Antigen
    CTSG
    Chargennummer
    022204892
    Validierte Anwendung
    Unfolding Profile
    Positivkontrolle
    ABIN3183694
    Negativkontrolle
    Bewertung

    Passed. ABIN3183694 showed Ti at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN3183694
    Sekundärantikörper
    Full Protocol
    • Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Anmerkungen

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C.
  • Target
    Cathepsin G (CTSG)
    Andere Bezeichnung
    Cathepsin G (CTSG Produkte)
    Synonyme
    CATG antikoerper, CG antikoerper, CTSG antikoerper, Ctsg antikoerper, cathepsin G antikoerper, CTSG antikoerper, Ctsg antikoerper, LOC505658 antikoerper, LOC509956 antikoerper, ctsg antikoerper, LOC100053921 antikoerper, LOC102174648 antikoerper, LOC100733619 antikoerper, LOC100154047 antikoerper
    Molekulargewicht
    22 kDa
    Gen-ID
    1511
    UniProt
    P08311
    Pathways
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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