Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Complement C4A/C4B (AA 1241-1256) Antikörper

Reaktivität: Human IHC (fro), IF Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN318426
  • Target
    Complement C4A/C4B
    Bindungsspezifität
    • 3
    • 2
    • 2
    AA 1241-1256
    Reaktivität
    • 9
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 4
    • 4
    • 1
    Kaninchen
    Klonalität
    • 5
    • 4
    Polyklonal
    Konjugat
    • 9
    Unkonjugiert
    Applikation
    • 8
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
    Spezifität
    This antibody is specific for complement split product C4d.
    Aufreinigung
    Purified Ig prepared by affinity chromatography on Protein G
    Immunogen
    Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.
    Isotyp
    IgG
  • Applikationshinweise
    Immunohistochemistry on frozen sections: 1/20 - 1/80. Immunohistochemistry on paraffin sections: 1/20 - 1/80, Heat induced epitope retrieval isrecommended. Immunofluorescence: 1/20 - 1/80.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 mg/mL
    Buffer
    Phosphate buffered saline containing 0.09 % Sodium Azide (NaN3)
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Complement C4A/C4B
    Synonyme
    C4 antikoerper, C4-1 antikoerper, C4b antikoerper, complement component 4A (Rodgers blood group) antikoerper, C4a antikoerper
    Hintergrund
    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.Synonyms: CPAMD2, CPAMD3, Complement Component 4
    Gen-ID
    720
    NCBI Accession
    NP_009224
    UniProt
    P0C0L4
Sie sind hier:
Kundenservice