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HA-Tag Antikörper
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Produktdetails anti-HA-Tag Antikörper
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Target
Alle HA-Tag Produkte
HA-Tag
Reaktivität
Bitte anfragen
Wirt
Alle Wirte für HA-Tag Antikörper
Kaninchen
Klonalität
Alle Klonalitäten für HA-Tag Antikörper
Polyklonal
Konjugat
Alle Konjugate für HA-Tag Antikörper
Dieser HA-Tag Antikörper ist unkonjugiert
Applikation
Alle Applikationen für HA-Tag Antikörper
Western Blotting (WB)
Sequenz
YPYDVPDYA
Aufreinigung
Immunoaffinity Chromatography using epitope-specific peptide.
Immunogen
The antiserum was produced by immunizing rabbit with synthesized peptide containing the Influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
Alternativen
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Anwendungsinformationen
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Applikationshinweise
Western Blot (1/500-1/1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50 % Glycerol, 0.02 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at-20 °C.
Referenzen für anti-HA-Tag Antikörper (ABIN319423)
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Qiu, Ding, Zou, Tan, Liu, Fu, Xu : "Divergent roles of amino acid residues inside and outside the BB loop affect human Toll-like receptor (TLR)2/2, TLR2/1 and TLR2/6 responsiveness. " in: PLoS ONE , Vol. 8 , Issue 4 , pp. e61508 , (2013 ) (PubMed ).
Giridharan, Cai, Vitale, Naslavsky, Caplan : "Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis. " in: Molecular biology of the cell , Vol. 24 , Issue 11 , pp. 1776-90, S1-15 , (2013 ) (PubMed ).
Details zu HA-Tag
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Target
HA-Tag
Andere Bezeichnung
HA Epitope Tag (YPYDVPDYA) (HA-Tag Produkte )
Substanzklasse
Tag
Hintergrund
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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