Nestin Antikörper (AA 178-399)

Produktnummer ABIN3201404

Produktdetails anti-Nestin Antikörper

Target: Nestin (NES)

Bindungsspezifität: AA 178-399

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Verwendungszweck: Monoclonal Antibody to Nestin (NES)

Sequenz: MGHHHHHHSG SEF- EGQSQQVGA PGLQAPQGLP EAIEPLVEDD VAPGGDQASP EVMLGSEPAM GESAAGAEPG PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP RKDLEEAGGL GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ GRAEALGKVE SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP LGFYLRSPTS PRWDPTGEQR PPPQGETGK

Spezifität: The antibody is a mouse monoclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

Kreuzreaktivität: Ratte

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Nestin (NES) corresdonding to Leu178~Pro399 with N-terminal His Tag

Klon: D4

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin (NES Produkte)