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PTH1R Antikörper (AA 146-169)

PTH1R Reaktivität: Human, Maus, Schwein, Rind (Kuh), Hund, Affe, Pferd WB, IHC, IHC (p) Wirt: Maus Monoclonal unconjugated
Produktnummer ABIN334275
  • Target Alle PTH1R Antikörper anzeigen
    PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))
    Bindungsspezifität
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 146-169
    Reaktivität
    • 42
    • 17
    • 11
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Schwein, Rind (Kuh), Hund, Affe, Pferd
    Wirt
    • 35
    • 6
    • 1
    Maus
    Klonalität
    • 36
    • 6
    Monoklonal
    Konjugat
    • 31
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PTH1R Antikörper ist unkonjugiert
    Applikation
    • 28
    • 18
    • 16
    • 9
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Recognizes human PTH/PTHrP receptor, MW 80kD, and the receptor dimer, Mr 160kD. A faint nonspecific band at 110kD is also detected. This antibody does not detect the PTH2 receptor.
    Homologie
    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%) Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%) Opossum, Chicken (92%) Xenopus (88%).
    Aufreinigung
    Protein G purified
    Immunogen
    Synthetic peptide corresponding to aa146-169 of human PTH/PTHrP Receptor [RRCDRNGSWELVPGHNRTWANYSE]. Species sequence Homology: Mouse & rat (23/24aa). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%), Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%), Opossum, Chicken (92%), Xenopus (88%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG1 kappa
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  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Detects PTH/PTHrP receptor in human kidney, vascular smooth muscle and ovary sections. Receptor was also detected in porcine oocytes. Western Blot: 0.5-2 μg/mL. Detects PTH/PTHrP receptor from RIPA lysates of 3T3-L1 pre-adipose fibroblasts. 3T3-L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P3109-50. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))
    Andere Bezeichnung
    PTHR / PTHR1 (PTH1R Produkte)
    Synonyme
    PFE antikoerper, PTHR antikoerper, PTHR1 antikoerper, PPR antikoerper, Pthr antikoerper, Pthr1 antikoerper, pthr1 antikoerper, zPTH1R antikoerper, PTHrel antikoerper, PTH1 antikoerper, PTH antikoerper, parathyroid hormone 1 receptor antikoerper, parathyroid hormone 1 receptor a antikoerper, PTH1R antikoerper, Pth1r antikoerper, pth1ra antikoerper
    Hintergrund
    Name/Gene ID: PTH1R
    Subfamily: Parathyroid hormone
    Family: GPCR

    Synonyms: PTH1R, Parathyroid hormone 1 receptor, Parathyroid hormone receptor 1, PFE, Pth-related peptide receptor, PTHR, PTH/PTHr receptor, Pth/pthrp receptor, Parathyroid hormone receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR1, PTHrP-R, PTHrP-receptor
    Gen-ID
    5745
    UniProt
    Q03431
    Pathways
    Regulation of Carbohydrate Metabolic Process
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