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GAD Antikörper (Internal Region)

Name: GAD Antikörper (Internal Region)
Brand: antibodies-online
SKU: ABIN334405
Price: 792.57 EUR
Availability: InStock

Verified GAD1 Reaktivität: Human WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated

ABIN334405 (0. (GAD Antikörper (Internal Region))

ABIN334405 (4µg/ml) staining of paraffin embedded Human Cerebellum. (GAD Antikörper (Internal Region))

IHC

ABIN334405 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). (GAD Antikörper (Internal Region))

3 Abbildungen

(1 reference)

Produktnummer ABIN334405

Datenblatt (PDF)

Target Alle GAD (GAD1) Antikörper anzeigen

GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
Bindungsspezifität
15

11

9

8

8

4

4

3

2

1

1

1

1

1

1

1

1
Internal Region
Reaktivität
96

61

56

6

6

4

4

3

2

2

1

1

1
Human
Wirt
85

15

3

1
Ziege
Passende Sekundärantikörper
Klonalität
82

22
Polyklonal
Konjugat
53

8

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GAD Antikörper ist unkonjugiert
Applikation
72

31

23

13

13

13

11

10

9

6

5

4

3

3

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck

GAD1 (isoform GAD67)

Sequenz

PDSPQRREKL HK

Spezifität

This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.

Kreuzreaktivität

Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Immunogen

Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.

Isotyp

IgG

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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-200) antibody
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 455-594) antibody
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibody
GAD1 Reaktivität: Human WB, IHC, ELISA, Coat, StM Wirt: Maus Monoclonal GAD1-2391 unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, ICC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibody
GAD1 Reaktivität: Human WB, ELISA, FACS, Coat Wirt: Maus Monoclonal GAD1-2563 unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 14-122) antibody
GAD1 Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 Reaktivität: Human WB, IHC, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Western Blot: Approx 65-70 kDa band observed in Human Cerebral Cortex lysates (calculated MW of 66.9 kDa according to NP_000808.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Magri, Giacopuzzi, La Via, Bonini, Ravasio, Elhussiny, Orizio, Gangemi, Valsecchi, Bresciani, Barbon, Vita, Gennarelli: "A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme." in: Scientific reports, Vol. 8, Issue 1, pp. 15470, (2018) (PubMed).
Target

GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Andere Bezeichnung

GAD1 (GAD1 Produkte)

Hintergrund

GAD1, glutamate decarboxylase 1 (brain, 67 kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25

Gen-ID

2571, 14415, 24379

NCBI Accession

NP_000808