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ITCH Antikörper (Internal Region)

Verified ITCH Reaktivität: Human ELISA, WB, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN334426
  • Target Alle ITCH Antikörper anzeigen
    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
    Bindungsspezifität
    • 11
    • 8
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 39
    • 36
    • 9
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 47
    • 4
    Ziege
    Klonalität
    • 46
    • 5
    Polyklonal
    Konjugat
    • 29
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ITCH Antikörper ist unkonjugiert
    Applikation
    • 35
    • 33
    • 8
    • 4
    • 3
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    ITCH / AIF4
    Sequenz
    EIKSHDLKPN GGN
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
    Andere Bezeichnung
    ITCH (ITCH Produkte)
    Synonyme
    AIF4 antikoerper, AIP4 antikoerper, NAPP1 antikoerper, dJ468O1.1 antikoerper, 6720481N21Rik antikoerper, 8030492O04Rik antikoerper, A130065M08 antikoerper, C230047C07Rik antikoerper, itchy E3 ubiquitin protein ligase antikoerper, itchy, E3 ubiquitin protein ligase antikoerper, ITCH antikoerper, Itch antikoerper
    Hintergrund
    ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui
    Gen-ID
    83737, 16396, 311567
    NCBI Accession
    NP_113671, NP_001244066, NP_001244067
    Pathways
    Activation of Innate immune Response, CXCR4-mediated Signaling Events
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