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COXII Antikörper

COXII Reaktivität: Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Mais (Zea mays) WB, BN PAGE Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN334535
  • Target Alle COXII Produkte
    COXII (Cytochrome Oxidase Subunit II (COXII))
    Reaktivität
    Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Mais (Zea mays)
    Wirt
    • 1
    Kaninchen
    Klonalität
    • 1
    Polyklonal
    Konjugat
    • 1
    Dieser COXII Antikörper ist unkonjugiert
    Applikation
    Western Blotting (WB), Blue-native PAGE (BN PAGE)
    Spezifität
    Arabidopsis thaliana (leaf extract and isolated mitochondria).
    Homologie
    dicots including Cucumis melo, Glycine max, Pisum sativum, monocots including Oryza sativa, moss Physcomitrella patens
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)
    Aufreinigung
    serum
    Immunogen
    KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
  • Applikationshinweise
    Recommended Dilution 1 : 1000 (WB), 1 : 1000 (BN-PAGE).
    Kommentare

    antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS pH 7.4
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

    Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

    Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

    Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).

  • Target
    COXII (Cytochrome Oxidase Subunit II (COXII))
    Andere Bezeichnung
    COXII (COXII Produkte)
    Synonyme
    cydB antikoerper, SCD78.13 antikoerper, qxtB antikoerper, cytochrome oxidase subunit II antikoerper, cytochrome d ubiquinol oxidase subunit II antikoerper, ubiquinol oxidase subunit II, cyanide insensitive antikoerper, COXII antikoerper, SCO3946 antikoerper, STM1793 antikoerper, cioB antikoerper, ctaC antikoerper, BPSS0234 antikoerper, appB antikoerper, BURPS1106A_A1873 antikoerper, PMI_RS06945 antikoerper, SSPA1008 antikoerper, SG1323 antikoerper, SEN1244 antikoerper, Sros_6663 antikoerper, VDBG_17011 antikoerper
    Hintergrund
    AGI Code: ATMG00160
    Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2
    Molekulargewicht
    expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)
    UniProt
    Q1XGA9, P04373
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