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TOP2 Antikörper (C-Term, N-Term)

TOP2 Reaktivität: Arabidopsis thaliana, Vicia faba WB, IL Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN334538
  • Target Alle TOP2 Antikörper anzeigen
    TOP2 (DNA Topoisomerase II (TOP2))
    Bindungsspezifität
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    C-Term, N-Term
    Reaktivität
    • 12
    • 2
    • 1
    Arabidopsis thaliana, Vicia faba
    Wirt
    • 11
    • 4
    Kaninchen
    Klonalität
    • 10
    • 5
    Polyklonal
    Konjugat
    • 10
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TOP2 Antikörper ist unkonjugiert
    Applikation
    • 15
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunolocalization (IL)
    Spezifität
    Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.
    Kreuzreaktivität (Details)
    Not reactive in: Nicotiana tabacum
    Homologie
    Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)
    Aufreinigung
    serum
    Immunogen
    The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
    Top Product
    Discover our top product TOP2 Primärantikörper
  • Applikationshinweise
    1: 2000 (WB), 1: 500 (IL)
    Kommentare

    Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 μL of sterile water
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

  • Target
    TOP2 (DNA Topoisomerase II (TOP2))
    Andere Bezeichnung
    TOP2 (TOP2 Produkte)
    Synonyme
    Topoii antikoerper, ptr11 antikoerper, DDBDRAFT_0191045 antikoerper, DDBDRAFT_0231510 antikoerper, DDB_0191045 antikoerper, DDB_0231510 antikoerper, CG10223 antikoerper, CHRAC antikoerper, Dmel\\CG10223 antikoerper, TOP2 antikoerper, TOPO II antikoerper, TOPOII antikoerper, TopII antikoerper, Topo II antikoerper, TopoII antikoerper, dCHRAC antikoerper, dTopoII antikoerper, top2 antikoerper, topo II antikoerper, topoII antikoerper, topoisomerase II antikoerper, DNA topoisomerase II antikoerper, DNA topoisomerase 2 antikoerper, DNA topoisomerase ii antikoerper, type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit antikoerper, Topoisomerase 2 antikoerper, TOP2 antikoerper, A583L antikoerper, top2 antikoerper, CNA03600 antikoerper, Tb09.160.4090 antikoerper, Tb11.01.3390 antikoerper, LMJF_28_2280 antikoerper, Top2 antikoerper
    Hintergrund
    AGI Code: At3g23890
    Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II
    Molekulargewicht
    expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)
    UniProt
    P30182
    Pathways
    Mitotic G1-G1/S Phases
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