S100A9 Antikörper (Internal Region)
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- Target Alle S100A9 Antikörper anzeigen
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser S100A9 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- S100A9
- Sequenz
- DTNADKQLSF EEF
- Kreuzreaktivität
- Human
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- C-DTNADKQLSFEEF
- Isotyp
- IgG
- Top Product
- Discover our top product S100A9 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 13-14 kDa band observed in Human Bone Marrow lysates and approx 14-15 kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2 kDa according to NP_002956.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour a
Peptide ELISA: antibody detection limit dilution 1:8000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended conc
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
- Andere Bezeichnung
- S100A9 (S100A9 Produkte)
- Hintergrund
- S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B
- Gen-ID
- 6280
- NCBI Accession
- NP_002956
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine
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