Basal Cell Cytokeratin Antikörper

Produktnummer ABIN335324

Produktdetails

Target: Basal Cell Cytokeratin

Reaktivität: Human, Kaninchen, Ratte, Meerschweinchen, Huhn, Hund, Hamster, Schwein, Wachtel, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Spezifität: RCK103 is a cytokeratin antibody recognizing (amongst others) cytokeratin 5. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium. Species reactivity: human, quail, chicken, rat, rabbit, hamster, canine, swine and guinea pig.

Aufreinigung: Purified

Immunogen: RCK103 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a mix of cell preparations containing human cytokeratins.

Klon: RCK103

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RCK103 is useful for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen

van Leenders, Dijkman, Hulsbergen-van de Kaa, Ruiter, Schalken: "Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 8, pp. 1251-8, (2000) (PubMed).

Vos, van den Ingh, de Neijs, van Mil, Ivanyi, Ramaekers: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." in: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, (1993) (PubMed).

Vos, van den Ingh, Ramaekers, Molenbeek, de Neijs, van Mil, Ivanyi: "The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region." in: Veterinary pathology, Vol. 30, Issue 4, pp. 352-61, (1993) (PubMed).

Verhagen, Ramaekers, Aalders, Schaafsma, Debruyne, Schalken: "Colocalization of basal and luminal cell-type cytokeratins in human prostate cancer." in: Cancer research, Vol. 52, Issue 22, pp. 6182-7, (1992) (PubMed).

Kuijpers, Tonnaer, Peters, Ramaekers: "Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig." in: Hearing research, Vol. 52, Issue 1, pp. 133-46, (1991) (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, (1990) (PubMed).

Schaafsma, Ramaekers, van Muijen, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract." in: Histochemistry, Vol. 91, Issue 2, pp. 151-9, (1989) (PubMed).

Verhagen, Aalders, Ramaekers, Debruyne, Schalken: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." in: The Prostate, Vol. 13, Issue 1, pp. 25-38, (1988) (PubMed).

Feitz, Debruyne, Vooijs, Herman, Ramaekers: "Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues." in: The Journal of urology, Vol. 136, Issue 4, pp. 922-31, (1986) (PubMed).

Antigendetails

Target: Basal Cell Cytokeratin

Hintergrund: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.