Desmin Antikörper

Produktnummer ABIN335360

Produktdetails anti-Desmin Antikörper

Target: Desmin (DES)

Reaktivität: Human, Maus, Ratte, Huhn, Hamster, Schwein, Hund, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Human, mouse, rat, rabbit, hamster, canine, chicken and swine.

Aufreinigung: Purified

Immunogen: RD301 is a mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal desmin extract of chicken gizzard.

Klon: RD301

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Desmin Antikörper (ABIN335360)

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, (2009) (PubMed).

Dispersyn, Geuens, Ver Donck, Ramaekers, Borgers: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, (2001) (PubMed).

Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Schaart, Viebahn, Langmann, Ramaekers: "Desmin and titin expression in early postimplantation mouse embryos." in: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Verhagen, Aalders, Ramaekers, Debruyne, Schalken: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." in: The Prostate, Vol. 13, Issue 1, pp. 25-38, (1988) (PubMed).

Quax, van den Broek, Egberts, Ramaekers, Bloemendal: "Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer." in: Cell, Vol. 43, Issue 1, pp. 327-38, (1986) (PubMed).

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: Desmin (DES Produkte)

Synonyme: DES antikoerper, des-b antikoerper, MGC80853 antikoerper, des antikoerper, csm1 antikoerper, csm2 antikoerper, desm antikoerper, cmd1i antikoerper, MGC75911 antikoerper, LOC100220724 antikoerper, desmin antikoerper, cb290 antikoerper, fb59a12 antikoerper, wu:fb59a12 antikoerper, zgc:109859 antikoerper, CSM1 antikoerper, CSM2 antikoerper, LGMD2R antikoerper, wu:fc11d08 antikoerper, zgc:154009 antikoerper, des-a antikoerper, MGC52614 antikoerper, desmin antikoerper, desmin, gene 1 L homeolog antikoerper, desmin, gene 1 antikoerper, desmin a antikoerper, desmin b antikoerper, desmin, gene 1 S homeolog antikoerper, desmin, gene 2 S homeolog antikoerper, DES antikoerper, des.1.L antikoerper, des.1 antikoerper, des antikoerper, desma antikoerper, Des antikoerper, desmb antikoerper, des.1.S antikoerper, des.2.S antikoerper

Hintergrund: Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.