Reticulon 1C Antikörper

Produktnummer ABIN335376

Produktdetails anti-Reticulon 1C Antikörper

Target: Reticulon 1C (NSP-C)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Spezifität: Human and rat.

Aufreinigung: Purified

Immunogen: RNL-4 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a BALB/c mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.

Klon: RNL-4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RNL-4 recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies. RNL-4 is useful for immunocytochemistry, immunohistochemistry on frozen tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Reticulon 1C Antikörper (ABIN335376)

Kim, Yoo, Broers, Cairns, Lubec: "Neuroendocrine-specific protein C, a marker of neuronal differentiation, is reduced in brain of patients with Down syndrome and Alzheimer's disease." in: Biochemical and biophysical research communications, Vol. 276, Issue 1, pp. 329-34, (2000) (PubMed).

Hens, Nuydens, Geerts, Senden, Van de Ven, Roebroek, van de Velde, Ramaekers, Broers: "Neuronal differentiation is accompanied by NSP-C expression." in: Cell and tissue research, Vol. 292, Issue 2, pp. 229-37, (1998) (PubMed).

Senden, Timmer, de Bruïne, Wagenaar, Van de Velde, Roebroek, Van de Ven, Broers, Ramaekers: "A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers." in: The Journal of pathology, Vol. 182, Issue 1, pp. 13-21, (1997) (PubMed).

Senden, Timmer, Boers, van de Velde, Roebroek, Van de Ven, Broers, Ramaekers: "Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A." in: European journal of cell biology, Vol. 69, Issue 3, pp. 197-213, (1996) (PubMed).

Details zu Reticulon 1C

Target: Reticulon 1C (NSP-C)

Andere Bezeichnung: Reticulon-1C / NSP-C

Hintergrund: Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).