Cofilin Antikörper (N-Term)

Produktnummer ABIN336102

Produktdetails anti-Cofilin Antikörper

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Bindungsspezifität: AA 1-166, N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: The antibody recognizes Human Cofilin-1. Other species not tested.

Produktmerkmale: Synonyms: CFL1, CFL, p18, Cofilin non-muscle isoform, 18 kDa phosphoprotein

Aufreinigung: Protein-G affinity chromatography

Immunogen: Recombinant human Cofilin-1 (aa 1-166) purified from E. coli

Klon: AT1C1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This Cofilin 1 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin-1 (CFL1 Produkte)

Hintergrund: Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

Gen-ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events