Cullin 1 Antikörper (C-Term)

Produktnummer ABIN337174

Produktdetails anti-Cullin 1 Antikörper

Target: Cullin 1 (CUL1)

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cullin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes Cullin 1. Reacts specifically with the ~86kD CUL-1 protein and does not cross-react with the related CUL-2 protein. This antibody is confirmed to be reactive with human. Reactivity is confirmed from extracts of IMR90 (human fibroblasts) and Wi-38 cells.

Aufreinigung: Protein A purified

Immunogen: A synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunoprecipitation: 5-10 μg/ IP reaction.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Cullin 1

Target: Cullin 1 (CUL1)

Andere Bezeichnung: Cullin 1 / CUL1 (CUL1 Produkte)

Hintergrund: Name/Gene ID: CUL1

Synonyms: CUL1, Cullin 1, Cullin-1, CUL-1

Gen-ID: 8454

UniProt: Q13616

Pathways: Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases, Regulation of Cell Size