HSPG2 Antikörper
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- Target Alle HSPG2 Antikörper anzeigen
- HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))
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Reaktivität
- Human, Rind (Kuh)
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser HSPG2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Marke
- IHC-plus™
- Spezifität
- Highly specific for bovine perlecan. The epitope is located in domain I. No cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: Human.
- Aufreinigung
- Protein A/G purified
- Immunogen
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Bovine corneal endothelial cells.
Type of Immunogen: Cells - Klon
- A71
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product HSPG2 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (1:4000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (1:50)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Kommentare
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Target Species of Antibody: Bovine
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))
- Andere Bezeichnung
- HSPG2 / Perlecan (HSPG2 Produkte)
- Hintergrund
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Name/Gene ID: HSPG2
Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS - Gen-ID
- 3339
- UniProt
- P98160
- Pathways
- Glycosaminoglycan Metabolic Process, Lipid Metabolism
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