PAR1 Antikörper (N-Term)

Produktnummer ABIN337370

Produktdetails anti-PAR1 Antikörper

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAR1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Marke: IHC-plus™

Spezifität: Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.

Aufreinigung: Protein A purified

Immunogen: A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.

Type of Immunogen: Synthetic peptide

Klon: ATAP2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), Flo (10 - 20 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 μg/mL. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 μg/mL. ELISA: 0.1-1.0 μg/mL.

Not recommended for: WB

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Andere Bezeichnung: F2R / Thrombin Receptor / PAR1 (F2R Produkte)

Synonyme: CF2R antikoerper, HTR antikoerper, PAR-1 antikoerper, PAR1 antikoerper, TR antikoerper, Par1 antikoerper, TRGPC antikoerper, AI482343 antikoerper, Cf2r antikoerper, ThrR antikoerper, Par-1 antikoerper, f2r-a antikoerper, par1 antikoerper, coagulation factor II thrombin receptor antikoerper, coagulation factor II (thrombin) receptor antikoerper, coagulation factor 2 (thrombin) receptor L homeolog antikoerper, F2R antikoerper, F2r antikoerper, f2r.L antikoerper

Hintergrund: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

Gen-ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus