COPB2 Antikörper (AA 494-511)

Produktnummer ABIN337394

Produktdetails anti-COPB2 Antikörper

Target: COPB2 (Coatomer Protein Complex, Subunit beta 2 (COPB2))

Bindungsspezifität: AA 494-511

Reaktivität: Human, Maus, Ratte, Fledermaus, Rind (Kuh), Hund, Pferd, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COPB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Detects rat coatomer-protein I beta (COP I beta).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%) Orangutan, Rabbit, Lizard (94%) Chicken (89%) Turkey, Xenopus, Sea anemone (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide: KVLAAQETHEGVTEDGIE, corresponding to amino acids 494-511 of Rat beta COP I. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%), Orangutan, Rabbit, Lizard (94%), Chicken (89%), Turkey, Xenopus, Sea anemone (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2 μg/mL, Detects an ~115kD protein representing COP I beta from PC-12 cell extract.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu COPB2

Target: COPB2 (Coatomer Protein Complex, Subunit beta 2 (COPB2))

Andere Bezeichnung: Beta-COP / COPB2 (COPB2 Produkte)

Hintergrund: Name/Gene ID: COPB2

Synonyms: COPB2, Betaprime-COP, Beta'-COP, Beta-coat protein, Beta-COP, p102, Coatomer beta' subunit, Coatomer subunit beta

Gen-ID: 9276

UniProt: P35606