AKT Substrate (pSer), (pThr) Antikörper

Produktnummer ABIN337521

Produktdetails

Target: AKT Substrate

Bindungsspezifität: pSer, pThr

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Spezifität: Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic phospho- Akt substrate peptides.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA (1:1000), IHC-P, IP (1:20), WB (1:1000)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

Antigendetails

Target: AKT Substrate

Substanzklasse: Chemical