Dieser Myc Tag Antikörper ist konjugiert mit Biotin
Applikation
Western Blotting (WB)
Sequenz
EQKLISEEDL
Spezifität
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc. Species cross-reactivity: Human. Other species cross-reactivity not tested.
Homologie
Percent identity by BLAST analysis: Mouse (100%) Human, Chimpanzee, Gorilla, Rat, Amoeba (91%) Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).
Aufreinigung
Protein G purified
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%), Human, Chimpanzee, Gorilla, Rat, Amoeba (91%), Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).
Type of Immunogen: Synthetic peptide - KLH conjugated
Usage: Suitable for use in Western Blot. Western Blot: 0.1-1 μg/mL detects Myc-tagged recombinant protein. COS transfected cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-myc tag, biotin conjugate (1 μg/mL). Proteins were visualized using a streptavidin-HRP conjugate and a chemiluminescence detection system. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
PBS, 0.05 % sodium azide, 30 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.