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Myc Tag Antikörper (AA 410-420)

WB, IP Wirt: Maus Monoclonal unconjugated

Produktnummer ABIN339758

Datenblatt (PDF)

Target Alle Myc Tag Produkte

Myc Tag
Bindungsspezifität
21

21

12

8

6

2

2

1
AA 410-420

Reaktivität

Bitte anfragen
Wirt
76

49

6

5

5

5

1

1
Maus
Passende Sekundärantikörper
Klonalität
78

58

10
Monoklonal
Konjugat
68

13

10

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Myc Tag Antikörper ist unkonjugiert
Applikation
110

53

43

37

26

24

20

18

15

11

11

6

6

5

4

4

4

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP)

Sequenz

EQKLISEEDL

Spezifität

Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Homologie

Percent identity by BLAST analysis: Mouse (100%) Human, Chimpanzee, Gorilla, Rat, Amoeba (91%) Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).

Aufreinigung

Protein G purified

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%), Human, Chimpanzee, Gorilla, Rat, Amoeba (91%), Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp

IgG1
anti-Myc Tag antibody
WB, IP, ELISA, IF Wirt: Maus Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reaktivität: Human WB, IP, IHC (p), FACS Wirt: Maus Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reaktivität: Human FACS Wirt: Maus Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reaktivität: Human WB, IP, IHC (p), FACS Wirt: Maus Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-Myc Tag antibody
WB, IP, ELISA, IF Wirt: Maus Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Wirt: Maus Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Wirt: Maus Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB Wirt: Ziege Polyclonal unconjugated

anti-Myc Tag antibody (Biotin)
WB, IP, ELISA, IHC Wirt: Kaninchen Polyclonal Biotin

Applikationshinweise

Approved: IP, WB

Usage: Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 μg/mL detects Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag. Lysates from NIH/3T3 cells transfected with either empty vector, PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLLRKRGST, or PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLNGST were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Myc Tag (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Myc-tagged PP2A A subunit (~63kD). Longer exposure demonstrating weak detection by anti-Myc Tag of the Myc-tagged protein containing the epitope tag sequence MEQKLISEEDLNGST. Immunoprecipitation: Reported that this antibody immunoprecipitates Myc-tagged protein from transfected cells.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Target

Myc Tag

Abstract

Myc Tag Produkte

Substanzklasse

Tag