Phosphothreonine Antikörper

Produktnummer ABIN340104

Produktdetails anti-Phosphothreonine Antikörper

Target: Phosphothreonine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphothreonine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Spezifität: Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL. Species cross-reactivity: species independent.

Aufreinigung: Immunoaffinity purified

Immunogen: Keyhole Limpet Hemocyanin (KLH)- conjugated phosphothreonine conjugates and Phosvitin.

Type of Immunogen: Purified protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. ELISA: 0.5 μg/mL (kinase assay). Western Blot: 2 μg/mL versus mouse brain lysate. Incubate at RT for 4 hours.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Produkte

Substanzklasse: Amino Acid