Ubiquitin Antikörper

Produktnummer ABIN340405

Produktdetails anti-Ubiquitin Antikörper

Target: Ubiquitin

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Recognizes ubiquitin, both conjugated and unconjugated. Reactivity has been shown in human, bovine, chicken, Drosophila and C. elegans.

Aufreinigung: Purified

Immunogen: Purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin (KLH).

Type of Immunogen: Hapten

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF (1:10), IHC, IHC-Fr, IHC-P, IP, WB (1:10)

Usage: Can be used for immunoassays, Western Blot, Immunofluorescence and Immunoprecipitation. Furthermore the antibody is useful for Immunohistology after heat-induced retrieval on both frozen and paraffin embedded sections. Immunohistology: 1:10. Immunofluorescence: 1:10. Western Blot: 1:10. For Western Blot it is recommended to use lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (  pH 7.5)) for 30-60 minutes at 4?C, followed by extensive PBS washing.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Ubiquitin

Target: Ubiquitin

Andere Bezeichnung: Ubiquitin (Ubiquitin Produkte)

Synonyme: CG11624 antikoerper, DmUb antikoerper, DmUbi-p63E antikoerper, Dmel\\CG11624 antikoerper, Ub antikoerper, Ubi-P63e antikoerper, Ubi-p antikoerper, Ubi63E antikoerper, Ubi63p antikoerper, Ubip antikoerper, Ubiq antikoerper, Ubp antikoerper, ubi antikoerper, ubi-p63E antikoerper, ubi63E antikoerper, CG18282 antikoerper, CG32744 antikoerper, CR32744 antikoerper, DmUbi-p5E antikoerper, Dmel\\CG32744 antikoerper, ubiquitin antikoerper, si:dz180g5.5 antikoerper, si:busm1-180g5.5 antikoerper, LOC100194618 antikoerper, UBIQ antikoerper, Ubiquitin antikoerper, LOC100282068 antikoerper, Ubiquitin-63E antikoerper, Ubiquitin-5E antikoerper, transmembrane and ubiquitin-like domain containing 2 antikoerper, polyubiquitin antikoerper, Putative ubiquitin family protein antikoerper, ubiquitin antikoerper, ubiquitin B antikoerper, UBiQuitin antikoerper, Ubiquitin antikoerper, Ubi-p63E antikoerper, Ubi-p5E antikoerper, tmub2 antikoerper, LOC100194618 antikoerper, pco110026 antikoerper, LOC100135798 antikoerper, UBB antikoerper, ubq-1 antikoerper, LOC100190766 antikoerper

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway