CD14 Antikörper (AA 9-13, AA 39-44)

Produktnummer ABIN343167

Produktdetails anti-CD14 Antikörper

Target: CD14

Bindungsspezifität: AA 9-13, AA 39-44

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Functional Studies (Func), Blocking Peptide (BP)

Spezifität: Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14

Kreuzreaktivität: Corynebacterium diphtheriae, Rind (Kuh), Pferd

Kreuzreaktivität (Details): horse and bovine CD14,inhibits binding of LPS to horse and bovine CD14. Binding titre at horse CD14 1:100 and inhibition titre 1:10.

Aufreinigung: Protein A affinity column

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Klon: BiG10

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: total inhibition of LPS-binding to CD14: 100 μg/mL. Binding titre at human CD14 transfected CHO-cells: more than 10.000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Referenzen für anti-CD14 Antikörper (ABIN343167)

Hackett, Flaminio, Fortier: "Analysis of CD14 Expression Levels in Putative Mesenchymal Progenitor Cells Isolated from Equine Bone Marrow." in: Stem cells and development, (2010) (PubMed).

Mérant, Breathnach, Kohler, Rashid, Van Meter, Horohov: "Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity." in: Veterinary immunology and immunopathology, Vol. 131, Issue 1-2, pp. 1-8, (2009) (PubMed).

Stelter, Pfister, Bernheiden, Jack, Bufler, Engelmann, Schuett: "The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms." in: European journal of biochemistry / FEBS, Vol. 236, Issue 2, pp. 457-64, (1996) (PubMed).

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14 (CD14 Produkte)

Synonyme: CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Hintergrund: Background: biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molekulargewicht: ~50kDa

Gen-ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades