CD14 Antikörper (AA 39-41, AA 43-44)

Produktnummer ABIN343169

Produktdetails anti-CD14 Antikörper

Target: CD14

Bindungsspezifität: AA 39-41, AA 43-44

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Spezifität: Soluble and membrane bound human CD14 The antibody blocks LPS binding to membrane bound CD14

Kreuzreaktivität: Corynebacterium diphtheriae, Rind (Kuh), Hund, Meerschweinchen, Pferd, Schwein

Kreuzreaktivität (Details): cattle++, swine ++, dog+ horse+, guinea pig +,Inhibits binding of LPS to CD14 of swine++, dog+, cattle +, dog+, more than titer 10.000.

Aufreinigung: Protein A affinity column

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Klon: BiG14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: binding titre at human CD14 transfected CHO-cells: 1:50.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.6

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Referenzen für anti-CD14 Antikörper (ABIN343169)

Knobloch, Feldmann, Wahl, Jungck, Behr, Stoelben, Koch: "Endothelin receptor antagonists attenuate the inflammatory response of human pulmonary vascular smooth muscle cells to bacterial endotoxin." in: The Journal of pharmacology and experimental therapeutics, (2013) (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, (2012) (PubMed).

Schromm, Reiling, Howe, Wiesmueller, Roessle, Brandenburg: "Influence of serum on the immune recognition of a synthetic lipopeptide mimetic of the 19-kDa lipoprotein from Mycobacterium tuberculosis." in: Innate immunity, (2009) (PubMed).

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14 (CD14 Produkte)

Synonyme: CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Hintergrund: Background: biG 14 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molekulargewicht: ~50kDa

Gen-ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades