CD14 Antikörper (AA 147-152)

Produktnummer ABIN343170

Produktdetails anti-CD14 Antikörper

Target: CD14

Bindungsspezifität: AA 147-152

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon)

Spezifität: Soluble and membrane bound human CD14. The antibody does'nt significant block LPS binding to membrane bound CD14.

Aufreinigung: Protein G affinity column

Immunogen: human Monocytes

Klon: BiG2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: no significant inhibition of LPS binding to CD14

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.7

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Referenzen für anti-CD14 Antikörper (ABIN343170)

Stelter, Loppnow, Menzel, Grunwald, Bernheiden, Jack, Ulmer, Schuett: "Differential impact of substitution of amino acids 9-13 and 91-101 of human CD14 on soluble CD14-dependent activation of cells by lipopolysaccharide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 11, pp. 6035-44, (1999) (PubMed).

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, (1998) (PubMed).

Neumeister, Faigle, Sommer, Zaehringer, Stelter, Menzel, Schuett, Northoff: "Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14." in: Infection and immunity, Vol. 66, Issue 9, pp. 4151-7, (1998) (PubMed).

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14 (CD14 Produkte)

Synonyme: CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Hintergrund: Background: biG 2 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molekulargewicht: ~50kDa

Gen-ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades