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CD14 Antikörper

CD14 Reaktivität: Maus ELISA Wirt: Maus Monoclonal biG57 unconjugated

Produktnummer ABIN343179

Datenblatt (PDF)

Target Alle CD14 Antikörper anzeigen

CD14
Reaktivität
254

44

40

27

24

23

23

23

20

17

16

15

14

14

13

10

8

7

6

5

5

2

1

1
Maus
Wirt
225

53

25

3

1
Maus
Passende Sekundärantikörper
Klonalität
252

54

1
Monoklonal
Konjugat
125

33

25

20

15

8

6

5

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD14 Antikörper ist unkonjugiert
Applikation
232

89

64

57

55

52

22

16

13

12

7

6

4

4

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1
ELISA

Spezifität

Recombinant mouse CD14. The antibody don't blocks LPS binding to membrane bound CD14.

Aufreinigung

Protein G affinity column

Immunogen

recombinant mouse CD14

Klon

BiG57

Isotyp

IgG1

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CD14 Reaktivität: Human FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reaktivität: Human IHC, ELISA, ICC Wirt: Maus Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human IHC, ELISA Wirt: Maus Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ELISA, IF Wirt: Maus Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ISt, StM Wirt: Maus Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Wirt: Maus Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 Biotin

Applikationshinweise

ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitute with bidistilled water

Konzentration

1 mg/mL

Buffer

PBS, pH 7.15

Konservierungsmittel

Without preservative

Lagerung

-20 °C,-80 °C
Target

CD14

Andere Bezeichnung

CD14 (CD14 Produkte)

Hintergrund

Background: biG 57 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molekulargewicht

~50kDa

Gen-ID

12475

NCBI Accession

NP_033971

UniProt

P10810

Pathways

TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades