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EGFR Antikörper (Tyr1173)

Name: EGFR Antikörper (Tyr1173)
Brand: antibodies-online
SKU: ABIN343717
Price: 392.4 EUR
Availability: InStock

EGFR Reaktivität: Human WB, FACS, IP Wirt: Maus Monoclonal EM-13 unconjugated

Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 inEGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions). (EGFR Antikörper (Tyr1173))

Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified antibody (concentration in sample 3 μg/mL, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining). (EGFR Antikörper (Tyr1173))

FACS

Immunoprecipitation ofEGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody (C)and anti-EGFR monoclonal mAb108 (D). (EGFR Antikörper (Tyr1173))

Immunoprecipitation ofEGFR from EGF-treated A431 cells byphosphospecific monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody(C) and anti-EGFR monoclonal mAb108 (D). (EGFR Antikörper (Tyr1173))

4 Abbildungen

Produktnummer ABIN343717

Datenblatt (PDF)

Target Alle EGFR Antikörper anzeigen

EGFR (Epidermal Growth Factor Receptor (EGFR))
Bindungsspezifität
39

38

31

31

30

28

27

26

25

22

21

18

16

16

16

15

15

15

11

11

10

9

9

9

8

8

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
Tyr1173
Reaktivität
756

299

287

25

25

24

20

14

8

3

2

2

1

1

1

1

1

1
Human
Wirt
580

177

14

13

5

1

1
Maus
Passende Sekundärantikörper
Klonalität
542

244

2
Monoklonal
Konjugat
410

58

29

22

20

18

18

17

16

16

16

12

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

2

1

1

1

1

1

1
Dieser EGFR Antikörper ist unkonjugiert
Applikation
535

245

149

149

129

102

100

93

82

46

37

34

34

19

11

8

6

4

4

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Spezifität

The mouse monoclonal antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on intracellular tyrosine 1173.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.

Klon

EM-13

Isotyp

IgG1

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Applikationshinweise

Western blotting: Recommended dilution: 1 μg/mL, positive control: EGF-stimulated A431, negative control: non-stimulated A431.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung

EGFR (EGFR Produkte)

Hintergrund

Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2

Gen-ID

1956

UniProt

P00533

Pathways

NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine