EIF3E Antikörper (C-Term)

Produktnummer ABIN349583

Produktdetails anti-EIF3E Antikörper

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody is specific for mouse eIF3S6/Int6 protein.  A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence.  Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.

Kreuzreaktivität: Human, Ratte (Rattus), Hund, Schaf, Affe

Produktmerkmale: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Aufreinigung: purified

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to eIF3S6/Int6 by western blotting in the appropriate cell lysate or extract.  This antibody is capable of detecting both over-expressed and endogenous eIF3S6/Int6.

Kommentare:

Gene Name: EIF3E

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.8 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Haltbarkeit: 3 months

Details zu EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Andere Bezeichnung: Eif3S6 Int6 (EIF3E Produkte)

Synonyme: EIF3-P48 antikoerper, EIF3S6 antikoerper, INT6 antikoerper, eIF3-p46 antikoerper, 48kDa antikoerper, Eif3s6 antikoerper, Int6 antikoerper, eIF3-p48 antikoerper, eif3-p48 antikoerper, eif3s6 antikoerper, eif3s6-A antikoerper, eIF3e antikoerper, eIF3-S6 antikoerper, eif3s6-B antikoerper, EIF3SE antikoerper, eif3s6b antikoerper, im:7147439 antikoerper, QtsA-18056 antikoerper, eukaryotic translation initiation factor 3 subunit E antikoerper, eukaryotic translation initiation factor 3, subunit E antikoerper, eukaryotic translation initiation factor 3 subunit E L homeolog antikoerper, eukaryotic translation initiation factor 3 subunit 6 antikoerper, eukaryotic translation initiation factor 3 subunit E S homeolog antikoerper, eukaryotic translation initiation factor 3, subunit E, a antikoerper, Eukaryotic translation initiation factor 3 subunit E antikoerper, eukaryotic translation initiation factor 3, subunit E, b antikoerper, EIF3E antikoerper, Eif3e antikoerper, eif3e antikoerper, eif3e.L antikoerper, LOC692936 antikoerper, eif3e.S antikoerper, eif3ea antikoerper, eif-3.E antikoerper, eif3eb antikoerper, LOC108348260 antikoerper, int6 antikoerper

Hintergrund: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.
Synonyms: eIFe antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, INT6 antibody, Viral integration site protein INT-6 homolog antibody

Gen-ID: 16341, 45476573

UniProt: P60229

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C