PKC beta Antikörper (Internal Region)

Produktnummer ABIN964887

Hersteller Produkt- Nr.: 600-401-a76s

Dieses Anti-PKC beta-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PKC beta in WB und ELISA. Geeignet für Human.

Kurzübersicht für PKC beta Antikörper (Internal Region) (ABIN964887)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PKC beta Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-a76s

Hersteller: Rockland

Verwendungszweck: Protein Kinase C Beta Antibody

Kreuzreaktivität (Details): This antibody is specific for human PKC beta protein.

Produktmerkmale: Synonyms: rabbit anti-Protein kinase C beta antibody, PKC ß, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract.  Lysates from U251 or BxPC3 cells are suggested for western blotting.

Western Blot Dilution: 1:1,000 - 1:2,000

ELISA Dilution: 1:75,000 - 1:80,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Andere Bezeichnung: PRKCB

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis.  PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption.  Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gen-ID: 5579, 47157322

UniProt: P05771

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling