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ybeY Antikörper (C-Term)

Rockland 600-401-a94 ybeY Reaktivität: Human ELISA, IHC, WB Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN349601
Hersteller Produkt- Nr.: 600-401-a94
  • Target Alle ybeY Antikörper anzeigen
    ybeY (YbeY Metallopeptidase (Putative) (ybeY))
    Bindungsspezifität
    C-Term
    Reaktivität
    Human
    Wirt
    • 5
    Kaninchen
    Klonalität
    • 5
    Polyklonal
    Konjugat
    • 2
    • 1
    • 1
    • 1
    Dieser ybeY Antikörper ist unkonjugiert
    Applikation
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Hersteller Produkt- Nr.
    600-401-a94
    Hersteller
    Rockland
    Verwendungszweck
    KSHV ORF57 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein.
    Produktmerkmale
    Synonyms: rabbit anti-KSHV ORF57 antibody, MTA, human herpesvirus 8 protein
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
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    Discover our top product ybeY Primärantikörper
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band 50-55 kDa in size corresponding to KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:5,000 - 1:8,000

    ELISA Dilution: 1:100,000 - 170,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.55 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    ybeY (YbeY Metallopeptidase (Putative) (ybeY))
    Andere Bezeichnung
    ORF57 (ybeY Produkte)
    Hintergrund
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport.  KSHV ORF57 is capable of establishing both lytic and latent replication cycles.  In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected.  In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material.  Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation.  Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions.  KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm.  Most HSV-1 genes are unspliced, by contrast, ORF57 is spliced gene, the protein is 455 amino acids in length and 50 kDa in size.
    Gen-ID
    4961525
    NCBI Accession
    YP_001129410
    UniProt
    Q2HR75
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