p130 Antikörper

Produktnummer ABIN349607

Produktdetails anti-p130 Antikörper

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reaktivität: Human, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p130 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues.  Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.

Kreuzreaktivität: Schimpanse

Produktmerkmale: Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.

Aufreinigung: affinity purified

Sterilität: Sterile filtered

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein.  A residue of cysteine was added to facilitate coupling.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody is suitable for ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding to pRb2/p130, and a band approximately 4.2kDa in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: RBL2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.39 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Haltbarkeit: 3 months

Details zu p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Andere Bezeichnung: pRb2 p130 (RBL2 Produkte)

Synonyme: RBL2 antikoerper, RBR2 antikoerper, RRB2a antikoerper, PRB2 antikoerper, RBR-2 antikoerper, Rb2 antikoerper, p130 antikoerper, P130 antikoerper, retinoblastoma-like 2 (p130) antikoerper, RB transcriptional corepressor like 2 antikoerper, related to retinoblastoma 2 antikoerper, RBL2 antikoerper, LOC541652 antikoerper, Rbl2 antikoerper

Hintergrund: Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.
Synonyms: 130 kDa retinoblastoma associated protein antibody, RBR2 antibody, Retinoblastoma like 2 antibody, Retinoblastoma like protein 2 antibody, Retinoblastoma Related Gene antibody

Gen-ID: 5934

UniProt: Q08999

Pathways: Zellzyklus, Mitotic G1-G1/S Phases