LGR4 Antikörper (Internal Region)

Produktnummer ABIN349637

Hersteller Produkt- Nr.: 200-301-b45

Produktdetails anti-LGR4 Antikörper

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LGR4 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-301-b45

Hersteller: Rockland

Verwendungszweck: LGR4 Antibody

Kreuzreaktivität (Details): This antibody is specific for human LGR4 protein.

Produktmerkmale: Synonyms: mouse anti-LGR4 antibody, mouse anti-LGR 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4

Aufreinigung: Anti-LGR4 purified from concentrated tissue culture supernate by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Immunogen Type: Conjugated Peptide

Klon: 6G8-B3-G5-C3

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-LGR4 monoclonal antibody has been tested by ELISA and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.13 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-LGR4 Antikörper (ABIN349637)

Yi, Xiong, Gong, Bellister, Ellis, Liu: "Analysis of LGR4 receptor distribution in human and mouse tissues." in: PLoS ONE, Vol. 8, Issue 10, pp. e78144, (2014) (PubMed).

Details zu LGR4

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Andere Bezeichnung: LGR4 (LGR4 Produkte)

Hintergrund: Background: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Gen-ID: 55366, 157694513

UniProt: Q8N537