NEFH Antikörper

Produktnummer ABIN349701

Produktdetails anti-NEFH Antikörper

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Human, Porcine, Rat

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules

Klon: NF-05

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: Capture antibody.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-NEFH Antikörper (ABIN349701)

Porchet, Probst, Dráberová, Dráber, Riederer, Riederer: "Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex." in: Neuroreport, Vol. 14, Issue 7, pp. 929-33, (2003) (PubMed).

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament heavy protein (NEFH Produkte)

Synonyme: NF-H antikoerper, NF200 antikoerper, Nfh antikoerper, mKIAA0845 antikoerper, NFH antikoerper, NEFH antikoerper, neurofilament heavy polypeptide antikoerper, neurofilament, heavy polypeptide antikoerper, neurofilament heavy antikoerper, MCYG_06231 antikoerper, Nefh antikoerper, NEFH antikoerper

Hintergrund: Neurofilament heavy,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFH, NEFH, Neurofilament heavy chain, CMT2CC

Gen-ID: 4744

UniProt: P12036