PRNP Antikörper

Produktnummer ABIN349704

Produktdetails anti-PRNP Antikörper

Target: PRNP (Prion Protein (PRNP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRNP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant human prion protein

Klon: EM-20

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 0.5 μg/mL, non-reducing conditions are essential.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-PRNP Antikörper (ABIN349704)

Dvorakova, Vranac, Janouskova, ?ernilec, Koren, Lukan, Nováková, Matej, Holada, ?urin Šerbec: "Detection of the GPI-anchorless prion protein fragment PrP226* in human brain." in: BMC neurology, Vol. 13, pp. 126, (2013) (PubMed).

Details zu PRNP

Target: PRNP (Prion Protein (PRNP))

Abstract: PRNP Produkte

Hintergrund: Prion protein,CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.,Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC

Gen-ID: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation