ATG4C
(Autophagy related 4C Cysteine Peptidase (ATG4C))
Reaktivität
Human
Wirt
Schaf
Klonalität
Polyklonal
Konjugat
Dieser ATG4C Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (IHC)
Spezifität
Specific for ATG4C.
Kreuzreaktivität
Human, Maus, Ratte
Kreuzreaktivität (Details)
Other species not yet tested.
Aufreinigung
IgG
Immunogen
A synthetic peptide from human cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3) conjugated to an immunogenic carrier protein has been used as the antigen.
ATG4C
Reaktivität: Human
WB, IF, ICC
Wirt: Kaninchen
Polyclonal
PE
Applikationshinweise
IHC, WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handhabung
Avoid freeze and thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Haltbarkeit
12 months
Target
ATG4C
(Autophagy related 4C Cysteine Peptidase (ATG4C))
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, liver and testis.,Mammalian Autophagy,ATG4C, autophagin-3, APG4C, AUTL1, AUTL3, APG4-C, FLJ14867, ATG4 autophagy related 4 homolog C (S. cerevisiae), Autophagy-related protein 4 homolog C, Autophagy-related cysteine endopeptidase 3, AUT-like 3 cysteine endopeptidase