A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.
IHC, WB. A concentration of 1-5 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handhabung
Avoid freeze and thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Haltbarkeit
12 months
Gengenbacher, Müller-Rudorf, Poggio, Gräßel, Dumit, Kreutmair, Lippert, Duyster, Illert: "Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues." in: International journal of molecular sciences, Vol. 20, Issue 16, (2020) (PubMed).
FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.,Neurodegeneration,38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase