Dieser Rho-related GTP-binding protein Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (IHC)
Spezifität
Specific for RhO family.
Kreuzreaktivität
Human, Maus, Ratte
Kreuzreaktivität (Details)
Other species not yet tested.
Aufreinigung
IgG
Immunogen
A synthetic peptide from human Rho-related GTP-binding proteins (RhOA, RhOB, RhOC, RhOF, RhOQ) and RAC1, RAC2, RAC3 and CDC42 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species.
RhO (pan)
Reaktivität: E. coli
ELISA
Wirt: Kaninchen
Polyclonal
HRP
Applikationshinweise
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handhabung
Avoid freeze and thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.,Rho Family,Rho-related GTP-binding protein RhOA, RhOB, RhOC, RhOF, RhOQ, RAC1, RAC2, RAC3, CDC42