Ubiquitin Antikörper

Produktnummer ABIN356467

Produktdetails anti-Ubiquitin Antikörper

Target: Ubiquitin

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody stains senile plaques and neurons.
Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.

Aufreinigung: Protein A Chromatography

Immunogen: Purified Human Ubiquitin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigenretriever, boiling tissue for 15-20 minutes in 10 mM citrate, pH 6.0, followed by cooling atRT for 15-20 minutes. Immunofluorescence. Recommended Positive Control: Human brain from Alzheimer's disease.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 3 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Ubiquitin

Target: Ubiquitin

Andere Bezeichnung: Ubiquitin (Ubiquitin Produkte)

Hintergrund: Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin, it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer's disease, lewy bodies in Parkinson's disease, pick bodies in Pick's disease, inclusions in motor neuron disease, Mallory's Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes.Synonyms: Polyubiquitin-B, UBB

Gen-ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway