ACSL4 Antikörper (AA 244-274)

Produktnummer ABIN357780

Produktdetails anti-ACSL4 Antikörper

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Bindungsspezifität: AA 244-274

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACSL4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific to ACSL4/FACL4 (Center).

Aufreinigung: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide between 244~274 amino acids from the center region of human FACL4.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu ACSL4

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Andere Bezeichnung: ACSL4 (ACSL4 Produkte)

Synonyme: acsl4 antikoerper, zgc:66186 antikoerper, ACSL4 antikoerper, ACS4 antikoerper, FACL4 antikoerper, LACS4 antikoerper, MRX63 antikoerper, MRX68 antikoerper, 9430020A05Rik antikoerper, AU018108 antikoerper, Facl4 antikoerper, Lacs4 antikoerper, Acs4 antikoerper, acs4 antikoerper, acsl3 antikoerper, facl4 antikoerper, lacs4 antikoerper, mrx63 antikoerper, mrx68 antikoerper, T32A16.20 antikoerper, T32A16_20 antikoerper, long-chain acyl-CoA synthetase 4 antikoerper, acyl-CoA synthetase long chain family member 4a antikoerper, acyl-CoA synthetase long-chain family member 4 antikoerper, acyl-CoA synthetase long chain family member 4 antikoerper, AcsL4 antikoerper, acyl-CoA synthetase long chain family member 3 antikoerper, Long-chain-fatty-acid--CoA ligase 4 antikoerper, acyl-CoA synthetase long-chain family member 4 S homeolog antikoerper, AMP-dependent synthetase and ligase family protein antikoerper, acsl4a antikoerper, ACSL4 antikoerper, acsL4 antikoerper, acsl3 antikoerper, acsl4 antikoerper, Acsl4 antikoerper, acsl4.S antikoerper, LACS4 antikoerper

Hintergrund: Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligase (FACL), converts free long chain fatty acids into fatty acyl-CoA esters, key intermediates in the synthesis of complex lipids. The FACL4 gene encodes a form of LACS and is expressed in several tissues, including brain. FACL4 cDNA from brain encodes a gene product that shows preference for arachidonic acid as a substrate when expressed in mammalian cells.1 The sequence of the predicted 670-amino acid human protein is 97 % identical to that of rat ACS4. FACL4 is highly expressed in adult human brain, especially in the cerebellum and hippocampus, similar to the mouse.2 A strong cytoplasmic staining was found in the Purkinje and granular cells of the cerebellum and the pyramidal layer of hippocampus, indicating that FACL4 is specifically expressed in neurons and not in glial cells. Two patients with Alport syndrome, elliptocytosis, and mental retardation carried a large deletion of the COL4A5 region that included FACL4.3 The absence of FACL4 might play a role in the development of mental retardation or other signs associated with Alport syndrome. Two point mutations, 1 missense and 1 splice site change, were reported in the FACL4 gene in 2 families with nonspecific mental retardation.2 Analysis of enzymatic activity in lymphoblastoid cell lines of affected individuals revealed low levels compared with normal cells, indicating that both mutations are null mutations.Synonyms: ACS4, FACL4, LACS4, Long-chain acyl-CoA synthetase 4, Long-chain-fatty-acid--CoA ligase 4

Molekulargewicht: 79188 Da

Gen-ID: 2182, 5874

UniProt: O60488